WMO Education and Training Programme

The ultrafiltration assay is a vital technique in the separation and concentration of macromolecules, widely used in biochemical and pharmaceutical research. This assay enables the efficient removal of small molecular weight impurities and solvents from samples, concentrating proteins, nucleic acids, or other macromolecules of interest. By employing semi-permeable membranes, the ultrafiltration assay selectively retains larger molecules while allowing smaller ones to pass through, ensuring high purity and recovery rates. This method is particularly useful for desalting, buffer exchange, and the preparation of samples for downstream analyses. The ultrafiltration assay is an essential tool in laboratory workflows, contributing to the accuracy and reliability of experimental results.