WMO Education and Training Programme

RNA In Situ Hybridization is a widely-applicable histology technique that utilizes a nucleic-acid based probe to localize to RNA sequences of interest, and allows for visualization of mRNA expression in cells or tissues. This technique can provide valuable special and temporal information regarding gene expression in situ, such as whether expression at the mRNA level is limited to a specific anatomic structure or cell type.

Creative Bioarray offers Custom RNA ISH and ACD Bio's RNAscope service. In situ Hybridization is a popular approach for the evaluation of changes in biomarker expression or a particular drug response effect in tissues, especially in the case of novel biomarkers where were robust, a well validated antibodies are not commercially available. We have extensive experience in the quantification of RNA expression on a cell by cell basis across a whole tissue section or within defined regions of interest.